Recommended books, papers, and video’s on Fluorescence Microscopy

  1. An excellent paper was recently published in Nature Methods on Fluorescence Microscopy. “Tutorial: guidance for quantitative confocal microscopy” in Nature Protocols, compiled by James Jonkman, Claire Brown, Graham Wright, Kurt Anderson and Alison North.  This paper, which somehow gradually transformed from a small practical guide into quite an epic, was spearheaded by James Jonkman and ended up as a collaboration by facility heads across three continents.   We hope that you will find it a useful addition to the reading material you hand out to your confocal microscopy trainees – obviously it cannot compare to our still-beloved Pawley confocal handbook but it is a lot quicker to read!  There will undoubtedly be parts that some of you won’t agree with – truth be told, we often disagreed amongst ourselves, ha ha, but we trust that these animated discussions led to a fairer and more representative consensus in the end!  We hope that this will provide some light reading for your facility users and other imaging scientists while they are tied to home, as well as a good introduction for future new microscopists.  It’s worth noting that much of the paper is not only useful for confocal microscopy – the sections on specimen preparation, choosing the right microscope, statistics, and pilot projects, among others, should also appeal to those foraying into other microscopy techniques.  In addition, we listed almost 100 references pointing people to more in depth reading on many of the topics.
  2. Fundamentals of Light Microscopy and Electronic Imaging, Murphy, D.B. John Wiley & Sons, NY, 2013 Link to PDF
  3. Molecular Fluorescence: Principles and Applications, 2nd Edition, Bernard Valeur
  4. Principles of Fluorescence Spectroscopy, 3rd Edition, Joseph Lakowicz
  5. Zeiss microscopy courses
  6. Nikon MicroscopyU
  7. Leica: Learn & Share: Microscopy Basics
  8. BiteSizeBio Microscopy and Imaging video/tutorials