March 2022 – Elyra SIM2 imaging/processing

The OBC is excited to announce that the Elyra 7 Lattice SIM now has SIM2! Lattice SIM² gives double the conventional SIM resolution and discriminate the finest sub-organelle structures, even those no more than 60 nm apart. With Lattice SIM², the Elyra 7 allows for both super-resolution and high-dynamic imaging – without the need for special sample preparation

Click here for more on ZEISS Elyra 7 with Lattice SIM²

Zeiss Elyra 7 with Lattice SIM with SMLM and TIRF

Click here for more information on the system and a link to the knowledge base for the Elyra 7.

The Ziss Elrya-7 Lattice SIM + 3D Localization System for Live Cell Lattice SIM Super-Resolution + 3D Localization
Elyra 7 at the OBC has the following features:

4 Laser lines: 405nm, 488nm, 561nm, 640nm

Dual Camera: 2  pco.edge 4.2 camera system is designed for highest quantum efficiency, 16 bit dynamic range, high frame rates, long exposure times and extremely low readout noise


10x – EC Plan-Neofluar 10x/0.30 WD=5.2

40x OIL: Plan-APO 40x/1.4 Oil DIC (UV) VIS-IR

40x Water:

63x OIL: Plan-Apo 63x/1.4 Oil Corr WD: 0.35

63x OIL for TIRF and SMLM: Alpha Plan-Apo 63x/1.46 Oil Corr WD=0.10

63X Water:

Piezo Stage for fast, smooth, repeatable motion over distances of up to 500 microns with sub-nanometer resolution

Funding for the microscope was provided by the Vice Chancellor for Research, the Provost Office, the School of Biological Sciences, and the Samueli School of Medicine.

B. subtilis bacteria treated with a fluorescent analogue of teixobactin, a recently reported antibiotic that can kill Gram-positive pathogens without detectable resistance. The antibiotic targets peptidoglycan precursors, which is reflected by the bright staining of division sites and side walls of the bacteria. Image taken on the Elyra 7 by Mike Morris, PhD from Professor James Nowick’s lab.