The OBC provides access to state-of-the-art light microscopes (confocal, mesoscale lightsheet, and Super Resolution), as well as software for 3D and 4D analysis. All instruments are accessible 24/7 to trained researchers of laboratories across the campus and the local research community. All researchers are trained by Dr. Adeela Syed with additional imaging support provided as needed.
March 2022 – Elyra SIM2 imaging/processing
The OBC is excited to announce that the Elyra 7 Lattice SIM now has SIM2! Lattice SIM² gives double the conventional SIM resolution and discriminate the finest sub-organelle structures, even those no more than 60 nm apart. With Lattice SIM², the Elyra 7 allows for both super-resolution and high-dynamic imaging – without the need for special sample preparation
Click here for more on ZEISS Elyra 7 with Lattice SIM²
October 2021 – LSM 900 with Airyscan 2
The OBC is excited to announce that the LSM 900 with Airyscan 2 is installed and ready for use! The LSM 900 Airyscan gives up 120nm resolution, can image in “multiplex” mode to scan 4x faster and has “sample navigator” for taking a quick overview image of your sample for tiling and multi-position imaging.
Click here for more on Airyscan 2 and other features of the LSM 900!
July 1st, 2021
Congratulations to Dr. Sunil Gandhi and Ricardo Azevedo (MSTP student) for being featured in the NIH Director’s Fourth of July blog post. It’s fun to watch!
This video makes use of the optical clearing technology and the Z1 light sheet microscope at the OBC! We’re all excited and proud to see the great science being done at UC Irvine being highlighted!
The Optical Biology Center is a Shared Resource Facility to provide researchers access to resources, including state of the art instrumentation and technical support, to conduct biomedical research.
All publications with data acquired from any of the equipment in the OBC must have the following cited in the acknowledgements sections: “This study was made possible in part through access to the Optical Biology Core Facility of the Developmental Biology Center, a shared resource supported by the Cancer Center Support Grant (CA-62203) and Center for Complex Biological Systems Support Grant (GM-076516) at the University of California, Irvine.”
Confocals: Zeiss LSM 780, LSM 900, Leica Sp8, LSM 700
Super resolution: Elyra 7 with Lattice SIM² and SMLM
Mesoscale Light-sheet: Zeiss Z1 (Cover image of whole mouse brain expressing Thy1-EGFP, ChAT-tdTomato and imaged on the Z1 lightsheet, is courtesy Ricardo Azevedo, MSTP and Professor Sunil Gandhi, PhD)
Software: Imaris 3D/4D, Imaris Stitcher, Arivis Vision 4D, Huygens SVI
For training contact Dr. Adeela Syed
SUPPORT FOR CONFOCAL USERS
Rahul Warrior PhD, OBC Director
Adeela Syed PhD, OBC Manager
Hours: ~9:30am to 4:00pm and by appt.