Obtain Quantitative Information About Your Cells and Individual Molecules

The sensitivity of LSM780 is quite simply outstanding. The GaAsP detector achieves 65 percent quantum efficiency compared to 25 percent typically by conventional PMT detectors. This results in accurate details and contrast-rich images of the challenging specimens you encounter in your live cell imaging.

The system’s illumination and detection design allows you to simultaneously acquire up to ten dyes. You excite any common fluorophore with up to eight different lasers, detecting the signals with the 32 channel GaAsP detector. LSM 780 is so sensitive

The LSM780 at the OBC also has:

  • 2-photon laser for 2-photon excitation and Second Harmonic Generation (SHG) imaging
  • Environmental Chamber for live cell imaging
  • A FLIM (Fluorescence Lifetime Microscopy) detector for studying molecules based on their fluorescence lifetime provides a way of studying things such as metabolic states, FLIM based FRET etc

3-D Reconstruction in Imaris of whole mount porcine tissue SHG collagen (at 880nm) shown in purple and autofluoresence shown in blue. Courtesy of Dr. Emon Heidari in Dr. Brian Wong’s lab
MTGO-YFP expression in the Drosophila Larval brain.  Image acquired using photon counting mode on the LSM780. Courtesy of Dr. Adeela Syed