The Optical Biology Core Facility (OBC), founded in 1994, is directed by Dr. J. Lawrence Marsh, Professor, Department of Developmental & Cell Biology, and is managed by Drs. Adeela Syed and Tatiana Krasieva. The Optical Biology Core Facility is comprised of 2 main components:
1- A self use facility located in 4443 McGaugh Hall at the UC Irvine Campus houses a Zeiss LSM 780 and LSM 700, and a Leica SP8 confocal microscope that can image live cells, fixed samples, model organisms, single molecules and anything in between. The Zeiss LSM 780 has a two photon laser for deep tissue imaging and both the LSM 780 and Leica SP8 have 6 single photon lasers for imaging all fluorophores (405nm, 458, 488, 514, 561, and 633nm). The LSM 700 has 2 detectors, and 4 laser lines (405, 488, 555, and 633). A FLIM (Fluorescence Lifetime Microscopy) detector for studying molecules based on their fluorescence lifetime provides a way of studying things such as metabolic states of cancer cells compared to normal cells and any situation that would typically employ FRET is also available on the LMS 780.
A Volocity image analysis software package is available on a high end work station in the McGaugh Hall facility and it is set up so it can be used from individual office computers. Training is provided and targeted workshops occur routinely. Users can sign up for time on the scopes 24hours/day, 7 days a week. Recharges are $20/hr for the Zeiss 780 and SP8 and $15/hr 700 respectively and half that for off-peak hours (i.e. outside of 8-6, M-F). Dr. Syed manages this facility and provides training and on-site trouble shooting as well as experimental advice.
2- The Laser Microbeam and Medical Program facility (LAMMP) is an NIBIB Biomedical Technology Resource Center located in the Beckman Laser Institute and Medical Clinic (BLIMC). This facility is available for collaborative use and protocol development using a number of microscopes and technologies being developed by that center. Information about the capabilities can be found at http://lammp.bli.uci.edu/. Some of their capabilities include Multi-Dimensional Microscopy (MDM) platforms for studying skin carcinomas, techniques to detect early melanoma non-invasively, and breast cancer screening using visible light (diffuse optical spectroscopy and imaging, DOSI) rather than X-rays. Dr. Krasieva manages this facility and is available for consultation and extended protocol development for investigators seeking new imaging applications. They are supported by extensive shop facilities that allow construction and modification of imaging platforms (http://lammp.bli.uci.edu/).